Methods in Molecular Biology, 2011, 726: 299-312
Cytotoxic assessment of carbon nanotube interaction with cell cultures
The field of nanotoxicology recently has emerged out of the need to systematically study the biocompatibility and potential adverse effects of novel nanomaterials. Carbon nanotubes (CNT) are one of the most interesting types of nanomaterials, and recently, their use in applications has dramatically increased. Their potential adverse impact on human health and the environment, however, have caused them to be viewed with apprehension in certain cases so further studies into their toxicology are justified. Current methodologies using cell culture (in vitro) models are unreliable and are not yet able to offer conclusive results about the toxicity profile of CNT. The need for reliable and rapid toxicity assays that will allow high throughput screening of nanotube materials is a prerequisite for the valid assessment of CNT toxicity. The assay described here was developed based on the pitfalls and drawbacks of traditionally used cytotoxicity assays.